Journal: International Journal of Experimental Pathology

Article Title: Apoptosis and fibrosis are early features of heart failure in an animal model of metabolic cardiomyopathy

doi: 10.1111/j.1365-2613.2009.00647.x

Figure Lengend Snippet: Relative mRNA expression rates obtained by cDNA microarray analysis

Article Snippet: Microarray analysis Applied Biosystems Mouse Genome Survey Arrays V1.0 (Applied Biosystems, Foster City, CA, USA) were used to determine the transcriptional profiles of total RNA samples from cardiac muscle of two male animals of each genotype.

Techniques: Expressing, Microarray

Journal: Cell Death & Disease

Article Title: C-type lectin receptor Dectin3 deficiency balances the accumulation and function of FoxO1-mediated LOX-1 + M-MDSCs in relieving lupus-like symptoms

doi: 10.1038/s41419-021-04052-5

Figure Lengend Snippet: A Analysis pipeline to identify Foxo1 as the downstream markers (left) and gene expression profiling of MDSCs (right). The mRNA of MDSCs was extracted and analyzed by gene expression profiling using a Mouse Transcriptome Array (v.1.0) (Affymetrix). B Top 10 genes ranked by degree after analysis of signal-net. C QPCR validation of microarray data. D Phosphorylated FoxO1 and nuclear transfer FoxO1 protein expression were evaluated by western blot analysis in MDSCs isolated from WT mice and Dectin3 −/− mice with lupus. E Flow cytometry analysis of the apoptosis level of MDSCs from WT and Dectin3 −/− mice with lupus. F Bim, Bcl2, and Bax protein expression were evaluated by western blot analysis in MDSCs isolated from WT mice and Dectin3 −/− mice with lupus. G Schematic diagram of BM-MDSC induction. H Flow cytometry analysis of the apoptosis level of MDSCs after interfering with si-FoxO1 in Dectin3 −/− BM-MDSCs and overexpression with pcDNA3.1-FoxO1 in WT BM-MDSCs. I , J Bim, Bcl2, and Bax protein expression were detected by western blot analysis in BM-MDSCs after interference or overexpression of FoxO1. K Schematic diagram of injection of AAV-FoxO1 and siRNA fragments. L P-FoxO1 and FoxO1 protein expression were detected by western blot analysis. M Flow cytometry analysis of the apoptosis level of MDSCs. N Flow cytometry analysis of Th17 and Treg cell differentiation. Data represent the mean scores ± SEM. * P ≤ 0.05, ** P ≤ 0.01, *** P ≤ 0.001; n = 3–5 in each group.

Article Snippet: The mRNA of MDSCs was extracted and analyzed by gene expression profiling using a Mouse Transcriptome Array (v.1.0) (Affymetrix).

Techniques: Expressing, Microarray, Western Blot, Isolation, Flow Cytometry, Over Expression, Injection, Cell Differentiation

Journal: Cell Death & Disease

Article Title: C-type lectin receptor Dectin3 deficiency balances the accumulation and function of FoxO1-mediated LOX-1 + M-MDSCs in relieving lupus-like symptoms

doi: 10.1038/s41419-021-04052-5

Figure Lengend Snippet: A Gene expression profiling of M-MDSCs. The mRNA of M-MDSCs was extracted and analyzed by gene expression profiling using a Mouse Transcriptome Array (v.1.0) (Affymetrix). B Gene expression profiling of different genes on the surface of M-MDSCs. C mRNA expression of Fcgr4, Fpr1, CXCR2, IL-1R2, and OLR1 in M-MDSCs of WT mice and Dectin3 −/− mice with lupus. D Gene expression profiling of differential transcription factors in M-MDSCs. E mRNA expression of Plscr1, Erg, Optn, Dach1, and FoxO1 in M-MDSCs of WT mice and Dectin3 −/− mice with lupus mice. F Analysis of the correlation between FoxO1 and OLR1 genes. G LOX-1 protein expression was detected by western blot analysis. H – J Representative flow cytometry results of G-MDSCs and M-MDSCs in each group and the statistical data of percentages of G-MDSCs and M-MDSCs in BM. K , L Representative flow cytometry results of the LOX-1 expression level in M-MDSCs in the BM of each group. M – O Representative flow cytometry results of G-MDSCs and M-MDSCs in each group and the statistical data of percentages of G-MDSCs and M-MDSCs in the spleen. P , Q Representative flow cytometry results of the LOX-1 expression level in M-MDSCs in the spleen of each group. Data represent the mean scores ± SEM. * P ≤ 0.05, ** P ≤ 0.01, and *** P ≤ 0.001; n = 3–5 in each group.

Article Snippet: The mRNA of MDSCs was extracted and analyzed by gene expression profiling using a Mouse Transcriptome Array (v.1.0) (Affymetrix).

Techniques: Expressing, Western Blot, Flow Cytometry